High Performance Liquid Chromatography (HPLC)

Overview

The High Performance Liquid Chromatography (HPLC) course focuses on one of the most widely used analytical techniques in forensic, pharmaceutical, and clinical laboratories. This course provides in-depth knowledge of chromatographic principles and their application in separating, identifying, and quantifying chemical compounds.

Learners gain a strong understanding of HPLC instrumentation, including pumps, injectors, columns, detectors, and data acquisition systems. The course explains mobile and stationary phases, method development, and optimization for different forensic and pharmaceutical applications.

Practical training emphasizes sample preparation, calibration, troubleshooting, and interpretation of chromatograms. Learners also understand validation parameters such as accuracy, precision, sensitivity, and reproducibility in compliance with regulatory standards.